GSM4880502: SW480M P65 ChIP-Seq rep 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RELA
Cell type
Cell type Class
Digestive tract
Cell type
SW 480
Primary Tissue
unresolved
Tissue Diagnosis
unresolved
Attributes by original data submitter
Sample
source_name
colon
chip antibody
cat no:33-9900, lot no: QJ216251, mouse monoclonal, Invitrogen
cell line
SW480
transfection
stably transfected with empty vector
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with DSG (20593, lot TF263080, Thermo Scientific) for 45 min and then incubated with formaldehyde (1%) for 10 minutes. Nuclear chormatin fractions were separted and immunoprecipitated with P65 anitboides. The libraries of ChIP DNA were prepared by using the kit NEB Next Ultra II DNA Library Prep Kit for Illumina following user instrcutions