Antigen

Antigen Class

TFs and others

Antigen

RELA

Cell type

Cell type Class

Digestive tract

Cell type

SW 480

Primary Tissue

unresolved

Tissue Diagnosis

unresolved

Sample

source_name

colon

chip antibody

cat no:33-9900, lot no: QJ216251, mouse monoclonal, Invitrogen

cell line

SW480

transfection

stably transfected with empty vector

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked with DSG (20593, lot TF263080, Thermo Scientific) for 45 min and then incubated with formaldehyde (1%) for 10 minutes. Nuclear chormatin fractions were separted and immunoprecipitated with P65 anitboides. The libraries of ChIP DNA were prepared by using the kit NEB Next Ultra II DNA Library Prep Kit for Illumina following user instrcutions

Sequencing Platform

instrument_model

NextSeq 550

hg38

Number of total reads

52122209

Reads aligned (%)

88.7

Duplicates removed (%)

2.8

Number of peaks

1616 (qval < 1E-05)

hg19

Number of total reads

52122209

Reads aligned (%)

88.1

Duplicates removed (%)

3.8

Number of peaks

1692 (qval < 1E-05)